Device

Part:BBa_K1491036:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-26)


Constitutive Dead ER Intein T7 RNAP with YFPH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425


Design Notes

Constitutive dead ER Intein T7 RNAP with YFP for quantification of T7 RNAP to use as control for WT Intein


Source

The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the dead ER Intein is BBa_K1491026, the YFP is BBa_K864100

References